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Functional analysis of M2 and ISE. (A) Partial sequences of exon 3B: M2 and ISE (small cases) are underlined. The mutations and the name of the constructs are shown (see text for details). (B) Results of RT-PCR assay of PLP and DM20 from differentiated Oli-neu cells transfected with wild-type PLP-neo (WT) and mutant constructs, M2-MT, ISEdel, M2-MT-ISEdel, ISE-ISE and M2F-M2F. This is a representative experiment (n = 2).
