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. 2007 Jun 6;35(12):3963–3973. doi: 10.1093/nar/gkm355

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Abundance of mRNA for selenoproteins, selenoprotein-synthesis factors, and housekeeping genes. Total RNA was extracted from brain (A), heart (B), intestine (C), kidney (D), liver (E), lung (F), spleen (G), and testes (H), which were used for cDNA synthesis and real-time PCR analysis. Levels of each target mRNA relative to hprt are shown for each tissue and grouped into either low copy (average <0.5) on the left, medium copy (average 0.5–10) in the middle and high copy (average >10) on the right. Number of mice used for each tissue were 3 (intestine, kidney, liver and spleen), 4 (brain and testes), 5 (heart) or 6 (lung) and results represent mean +SE.

Figure 1. — Abundance of mRNA for selenoproteins, selenoprotein-synthesis factors, and housekeeping genes. Total RNA was extracted from brain (A), heart (B), intestine (C), kidney (D), liver (E), lung (F), spleen (G), and testes (H), which were used for cDNA synthesis and real-time PCR analysis. Levels of each target mRNA relative to hprt are shown for each tissue and grouped into either low copy (average <0.5) on the left, medium copy (average 0.5–10) in the middle and high copy (average >10) on the right. Number of mice used for each tissue were 3 (intestine, kidney, liver and spleen), 4 (brain and testes), 5 (heart) or 6 (lung) and results represent mean +SE.