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. 2007 Jun 6;35(12):3963–3973. doi: 10.1093/nar/gkm355

Figure 2.

Figure 2.

Comparison of Sel P knockout to wild-type mice for abundance of mRNA for selenoproteins, selenoprotein-synthesis factors and housekeeping genes. Total RNA was extracted from heart (A), lung (B), brain (C), and testes (D), which were used for cDNA synthesis and real-time PCR analysis. Levels of each target mRNA relative to hprt are shown for each tissue and grouped into either low copy (average <0.5) on the left, medium copy (average 0.5–10) in the middle and high copy (average >10) on the right. Black bars represent wild-type mice and white bars represent Sel P knockout mice. Number of mice used for each wild-type tissue were, 4 (brain and testes), 5 (heart) or 6 (lung) and for all 3 knockout tissues. Results represent mean +SE.