Figure 3.

Comparison of GPX1, GPX4, and Sel W levels in Sel P knockout versus wild-type mice. (A) Protein was extracted from heart, lung, brain and testes, which were then used for western blot analysis. Rabbit polyclonal antibodies were used to detect GPX1, GPX4 and Sel W for three Sel P knockout mice and three wild-type mice for each tissue. Equivalent protein loading was determined using α-tubulin for heart and lung and β-actin for brain and testes. (B) Densitometry was used to quantify western blot data as described in the Methods section. Results represent mean ±SE. Statistical significance was determined by student's t-test (*P < 0.05) Black and white bars represent wildtype (N=3) and knockout (N=3) mice, respectively.