Table 1.

Oligonucleotide primers used for real-time PCR

Name of target sequence	GenBank accession number			PCR product length
hprt	NM_013556.2	fwd	TCCTCCTCAGACCGCTTTT	90
		rev	CCTGGTTCATCGCTAATC
gapdh	NM_001001303	fwd	TGACATCAAGAAGGTGGTGAAGC	203
		rev	CCCTGTTGCTGTAGCCGTATTC
actin	NM_007393.1	fwd	TGACAGGATGCAGAAGGAGA	75
		rev	CGCTCAGGAGGAGCAATG
ubc	NM_019639.3	fwd	GAGGGCATCTCCCCTGAC	60
		rev	GCCATCTTCCAGCTGCTT
gpx1	NM_008160.2	fwd	ACAGTCCACCGTGTATGCCTTC	238
		rev	CTCTTCATTCTTGCCATTCTCCTG
gpx2	NM_030677.1	fwd	GTTCTCGGCTTCCCTTGC	64
		rev	TCAGGATCTCCTCGTTCTGAC
gpx3	NM_008161.1	fwd	ATTTGGCTTGGTCATTCTGG	105
		rev	CCACCTGGTCGAACATACTTG
gpx4	NM_001037741.1	fwd	TCTGTGTAAATGGGGACGATGC	174
		rev	TCTCTATCACCTGGGGCTCCTC
sel H	NM_001033166.2	fwd	GGAAGAAAGCGTAAGGCGGG	191
		rev	GGTTTGGACGGGTTCACTTGC
sel I	NM_027652.1	fwd	CCTGACATACTTCGACCCTGA	63
		rev	CAGTCAGGCACATGCTTATGA
sel K	NM_019979.1	fwd	TCCACGAAGAATGGGTAGGA	94
		rev	GCTTCTCAGAGCAGACATTTACCT
sel M	NM_053267	fwd	TGACAGTTGAATCGCCTAAAGGAG	113
		rev	AACAGCACGAGTTCGGGGTC
sel N	NM_029100.1	fwd	ACCTGGTCCCTGGTAAAGGAGC	177
		rev	GGTGATGTCAAGGAAGTAGTTGGC
sel O	NM_027905.2	fwd	GGCTGCCCATACCTGTGA	240
		rev	CGTGCTGTTCGCTGTGTC
sel P	NM_009155.2	fwd	CCTTGGTTTGCCTTACTCCTTCC	199
		rev	TTTGTTGTGGTGTTTGTGGTGG
sel R	NM_013759.1	fwd	TTCGTCCCTAAAGGCAAAGA	62
		rev	CATTCGCAGTCCATGTCCTA
sel S	NM_024439.2	fwd	CAGAAGATTGAAATGTGGGACAGC	116
		rev	CCTTTGGGGATGACAGATGAAGTAG
sel T	XR_004549.1	fwd	TGAGGCTCCTGCTGCTTC	110
		rev	GGTGGCGTACTGCATCTTTAAT
sel V	NM_175033.3	fwd	CAGGAGGAAGCATGCAAGA	62
		rev	TGGTTTGGCAGCATTTCAG
sel W	NM_009156.2	fwd	GGTGCCTCCCCAGAATCTAC	136
		rev	TGGGGGAATTCAGAGAGAGA
sps1	NM_175400.5	fwd	TGAACTGAAAGGCACAGGCTGC	143
		rev	CGCAAGTATCCATCCCAATGC
sps2	NM_009266.2	fwd	ACCGACTTCTTTTACCCCTTGG	166
		rev	TCACCTTCTCTCGTTCCTTTTCAC
tr1	NM_015762.1	fwd	CCTATGTCGCCTTGGAATGTGC	244
		rev	ATGGTCTCCTCGCTGTTTGTGG
tr2	NM_013711.1	fwd	GTTCCCCACATCTATGCCATTG	114
		rev	GGTTGAGGATTTCCCAAAGAGC
tr3	NM_153162.2	fwd	CTTTGCAAGATGCCAAGAAA	69
		rev	TCATGGCCTCCCAGTTGT
dio1	NM_007860.2	fwd	GCCTCTCAGGACAGAAGTGC	73
		rev	CTGCCAAAGTTCAACACCAG
dio2	NM_010050.1	fwd	CTGCGCTGTGTCTGGAAC	67
		rev	GGAGCATCTTCACCCAGTTT
dio3	NM_172119.1	fwd	GGAGGTTGTCCGACCTGAT	60
		rev	GTCCCTTGTGCGTAGTCGAG
sep15	NM_053102.1	fwd	GCTGTCAGGAAGAAGCACAA	74
		rev	TTTTCATCCGCAGACTTCAA
sbp2	XM_127336.5	fwd	GAAGCAGAGGGAGATACCTAAGGC	110
		rev	GGCTCACAGCACTTTCTTGGAG
secp43	NM_027925.2	fwd	ACCGTGATGAGCGTCAAAAT	74
		rev	GCGAATTCCACAAAGCAGTAG