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(A) CD spectra for 0.02 mg/ml DNase I in the absence (dotted line) and presence (solid line) of 20% (w/v) PEG 4000 at 25°C. (B) Thermal denaturation curves for DNase I in the absence (dotted line) and presence (solid line) of 20% (w/v) PEG 4000. The changes in the ellipticity were monitored at 222 nm. All measurements were performed in 50 mM HEPES (pH 7.2).
