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. 2007 Jun 13;35(12):4203–4213. doi: 10.1093/nar/gkm388

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Pre-rRNA processing in S. cerevisiae. (A) Structure and processing sites of the 35S pre-rRNA. This precursor contains the sequences for the mature 18S, 5.8S and 25S rRNAs that are separated by two internal transcribed spacer sequences, ITS1 and ITS2, and flanked by two external transcribed spacer sequences, 5′ ETS and 3′ ETS. The mature rRNA species are shown as bars and the transcribed spacer sequences as lines. The processing sites and the various probes used are indicated. (B) Schematic representation of the pre-rRNA processing pathway of the 35S pre-rRNA and pre-5S rRNA. Cleavage and trimming reactions are indicated. The data presented in this study suggest that Rpl3p is required for stability of the 27S pre-rRNAs. For reviews on pre-rRNA processing and the known processing enzymes, see (6,8).