Figure 4.
Effects of Rpl3p depletion on steady-state levels of 27S pre-rRNAs species. The same RNA samples described in the legend of the Figure 3 were used for primer extension analysis. Probe f (see Figure 1A for its location in the 35S pre-rRNA) was labelled and used for the reactions. Note that this probe allows detection of 27SA2 (as the stop at site A2), 27SA3 (as the stop at site A3), 27SB and 7S pre-rRNAs (as stops at sites B1L and B1S).
