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. 2007 May 30;35(12):3907–3917. doi: 10.1093/nar/gkm374

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — (A) CMV (+PPT)/HeLa cell lines contain bi-directional CMV promoter that allows expression of two reporter genes [firefly luciferase (luc) and GFP]. The PPT-wt sequence (5′AAAAGAAAAGGGGGGA3′) was inserted upstream of the luc gene and a mutated version (PPT-mut, 5′AAAAGAAGGGGAGGAA3′, the four mutations are underlined) was inserted upstream of the GFP gene. (B) PNA tridecamers are efficient inhibitors of translation. Cells were reversibly permeabilized by SLO in the presence of various PNAs (see ‘Materials and Methods’ section). PNA concentrations in final culturing conditions are indicated.