Table 2.
RNA-III* | wt | mut | |||||||
---|---|---|---|---|---|---|---|---|---|
PNA* | Tdis | Tass | Δa | Tdis | Tass | Δb | Tdis | Tass | Δa |
13mer-I | 88 | 70 | −18 | 78 | 65 | −13 | 83 | 58 | −25 |
13mer-I-Acr | 85 | 75 | −8 | 86 | 65 | −11 | |||
13mer-II | 52 | 52 | 0 | 45 | 45 | 0 | |||
11mer-bis | 87 | 80 | −7 | 74 | 69 | −5 | |||
9mer | 84 | 58 | −26 | 75 | 60 | −15 | |||
9mer-Acr | 86 | 59 | −27 | ||||||
9mer-bis | 82 | 78 | −4 |
*PNA and *RNA sequences in Table 1.
aΔ and bΔ = Tass − Tdis obtained in cooling–melting experiments with heating rates of 0.5°C/min and 0.1°C/min, respectively.