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. 2007 May 30;35(12):3907–3917. doi: 10.1093/nar/gkm374

Table 2.

UV-cooling-melting temperatures: temperatures of dissociation (Tdis) and association (Tass) in°C

RNA-III* wt mut


PNA* Tdis Tass Δa Tdis Tass Δb Tdis Tass Δa
13mer-I 88 70 −18 78 65 −13 83 58 −25
13mer-I-Acr 85 75 −8 86 65 −11
13mer-II 52 52 0 45 45 0
11mer-bis 87 80 −7 74 69 −5
9mer 84 58 −26 75 60 −15
9mer-Acr 86 59 −27
9mer-bis 82 78 −4

*PNA and *RNA sequences in Table 1.

aΔ and bΔ = TassTdis obtained in cooling–melting experiments with heating rates of 0.5°C/min and 0.1°C/min, respectively.