Figure 6.

Effects of DNA and σ⁷⁰ substitutions on equilibrium and kinetics of stable complex formation. (A) K_d. The asterisks above two of the bars are to indicate that these are lower estimates for the values of K_d. (B) k_on (calculated from values of k_obs and k_off). (C) Half lives, t_1/2, of the complexes, as calculated from the k_off values: k_off = 0.010, 0.031, 0.015 and 0.022 min⁻¹ respectively for complexes of Duplex with WT, W433L, Y430A and Y430F RNAP, and 0.134, 0.469, 0.049 and 0.326 min⁻¹ respectively for complexes of −11 2AP Duplex with WT, W433L, Y430A and Y430F RNAP. See the legend to Figure 5 and the Materials and Methods for experimental detail and calculation of k_on and t_1/2.