Figure 2.

Ear-2 represses transactivation mediated by PEBP2αB1. A Jurkat T cell line was transfected with 1 μg of MCSF-R-luc, 2 μg of pEF-PEBP2αB1, 2 μg of MSV-C/EBP, 1 μg of pEF-PEBP2β2, 0.5 μg of RSV-β-gal, and increasing amounts of pCMX-ear-2. Lanes: 1, 7, 13, and 19, 0 μg; 2, 8, 14, and 20, 1 μg; 3, 9, 15, and 21, 2 μg; 4, 10, 16, and 22, 4 μg; 5, 11, 17, and 23, 8 μg; 6, 12, 18, and 24, 12 μg. Luciferase activities were normalized to the β-galactosidase activities of each extract. Each value represents the mean of three independent experiments. Standard deviations are indicated by error bars.