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. 1998 Feb 17;95(4):1812–1817. doi: 10.1073/pnas.95.4.1812

Figure 6.

Figure 6

Northern blot analysis of PEBP2αB1 and ear-2 mRNA. Poly(A)+ RNAs from 32Dcl3 cells stably transfected with pBabeneo-ear-2 (A) or pBabeneo-AML1/ETO(MTG8) (B) were analyzed by Northern blotting using 5 μg or 2.5 μg of poly(A)+ RNA for the expression of ear-2 or PEBP2αB, respectively. Numbers indicate days in the medium containing G-CSF. Of the three bands representing the ear-2 mRNA in A, top two correspond to the exogenous and the bottom one to the endogenous mRNA. As an internal control, β-actin mRNA expression was analyzed for each membrane after stripping. The positions of 18S and 28S rRNA are indicated. AML1/ETO(MTG8) RNA was not detected by the labeled PEBP2αB1 probe in B Right. Expression of AML1/ETO(MTG8) was confirmed, however, by using the AML1/ETO(MTG8) cDNA as a probe (data not shown).