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. 2007 Jun 1;35(12):3945–3952. doi: 10.1093/nar/gkm357

Figure 5.

Figure 5.

Induction of TetR mutants by Tip variants. Induction of TetR mutants by basal expression levels (absence of IPTG) of TrxA-XTip variants is shown. X represents any of the 20 amino acids as depicted in one-letter abbreviations (see upper right insert for the sequence). TetR variants are encoded by pWH527-derivatives. Induction of TetR variants is indicated by the corresponding β-Gal activities expressed by the screening strain. White bars show the induction values of TetR-N82A, gray bars those of TetR-F86A and black bars those of TetR-N82A-F86A. Induction by the initially analysed M insertion is highlighted by a black box. Tip indicates induction by basal expression levels of TrxA(C)-Tip and the one-letter abbreviations indicate the inserted residue. The insert in the upper left corner shows the coding of the bars. The central insert shows Western blots indicating the steady-state protein levels of TetR, TetR-N82A, TetR-F86A and TetR-N82A-F86A. The molecular mass of the TetR variants is indicated on the left.