Table 1.
TetR-inducing protein | β-Gal activity (%) | |
---|---|---|
Repression | Induction | |
TrxA(C)-Tip | 2.3 ± 0.1 | 24 ± 2 |
TrxA(C)-MTip | 2.6 ± 0.1 | 37 ± 3 |
TrxA(C)-XTip | ||
X = Glycine | 3.5 ± 0.4 | 57 ± 5 |
Alanine | 3.7 ± 0.2 | 63 ± 4 |
Serine | 2.7 ± 0.1 | 41 ± 3 |
Threonine | 2.4 ± 0.2 | 34 ± 3 |
Cysteine | 2.4 ± 0.2 | 36 ± 3 |
Valine | 2.2 ± 0.1 | 6.9 ± 0.5 |
Isoleucine | 2.6 ± 0.2 | 8.7 ± 1.2 |
Leucine | 2.1 ± 0.1 | 6.3 ± 0.6 |
Aspartic Acid | 2.2 ± 0.1 | 2.0 ± 0.1 |
Asparagine | 3.1 ± 0.1 | 46 ± 4 |
Glutamic Acid | 1.9 ± 0.2 | 2.3 ± 0.2 |
Glutamine | 3.8 ± 0.1 | 64 ± 3 |
Lysine | 2.1 ± 0.1 | 2.2 ± 0.1 |
Arginine | 2.0 ± 0.1 | 2.2 ± 0.1 |
Histidine | 3.4 ± 0.1 | 57 ± 4 |
Phenylalanine | 3.3 ± 0.2 | 38 ± 1 |
Tyrosine | 2.3 ± 0.1 | 21 ± 1 |
Tryptophan | 2.7 ± 0.1 | 55 ± 1 |
Proline | 2.0 ± 0.1 | 1.5 ± 0.4 |
β-Gal activities were determined in E. coli DH5α(λtet50) expressing lacZ under Tet-control. tetR is encoded by pWH527. Expression of the C-terminal TrxA-Tip fusions was induced with 60 µM IPTG to determine peptide-based induction of TetR.