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Monitor RNase H cleavage of RNA in LNA-MB-E3/RNA duplex (left) and in DNA-MB/RNA duplex (right) using Ion Exchange HPLC. The concentrations of MBs and RNA were 1 µM. All samples were incubated overnight at room temperature before analyzed by ion-exchange HPLC (Dionex DNAPac™ PA-100 column (4 × 250 mm), 30%–70%, 45 min gradient 1 M NaCl/20 mM NaOH, pH 12).
