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Hybridization of DNA-MB and LNA-MB-E0 to 10-fold excess of loop cDNA (GCG ACC ATA GTG ATT TAG A). Both MBs shared the same sequence FAM-CCTAGCTCTAAATCACTATGGTCGCGCTA GG-DABCYL. DNA-MB was synthesized with DNA bases, while the LNA-MB-E0 was fully modified with LNA bases. The hybridization experiments were performed at room temperature in 20 mM Tris-HCl (pH 7.5) buffer containing 5 mM MgCl2 and 50 mM NaCl.
