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Hybridization of 100 nM MB-LNA-E3 to 500 nM loop cDNA(GCG ACC ATA GTG ATT TAG A) (blue) and shared-stem cDNA(CCT AGC GCG ACC ATA GTG ATT TAG A) (red). The sequence of MB-LNA-E3 was FAM-CCTAGC TCTAAATCACTATGGTCGCGCTAGG-DABCYL, with red letters representing LNA bases. The hybridization experiments were performed at room temperature in 20 mM Tris-HCl (pH 7.5) buffer containing 5 mM MgCl2 and 50 mM NaCl.
