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. 2007 Jun 18;35(12):e85. doi: 10.1093/nar/gkm433

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — (a) PCR of the GAPDH gene using DNA extracted from liver (L), spleen (S) and colon (C) tissue fixed in NBF, Z7 and stored in paraffin blocks at RT for 14 months (Old), compared with DNA extracted from liver (L), spleen (S) and colon (C) tissue fixed in NBF, Z7 and stored in paraffin blocks at RT for less than a week (New). Fresh-frozen samples (Frozen) tissues were used as positive control and a water only sample (H₂O) as negative control. (b) PCR amplification of the β-actin gene using DNA extracted from liver (L), spleen (S) and colon (C) tissue fixed in NBF, Z7 and paraffin blocks stored at RT for 14 months (Old), compared with DNA extracted from liver (L), spleen (S) and colon (C) tissue fixed in NBF, Z7 and stored in paraffin blocks at RT for less than a week (New). Fresh-frozen samples (Frozen) tissues were used as positive control and a water only sample (H₂O) as negative control. (c) Real-Time PCR showing amplification of the GAPDH gene using DNA extracted from liver, spleen and colon tissue fixed in NBF, Z7 and stored in paraffin blocks at RT for 14 months (Old), compared with DNA extracted from liver, spleen and colon (C) tissue fixed in NBF, Z7 and stored in paraffin blocks at RT for less than a week (New). Fresh-frozen samples (Frozen) tissues were used as positive control and a water only sample (H₂O) as negative control. Bars indicate the ±SEM.