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. 2007 Jun 18;35(12):e88. doi: 10.1093/nar/gkm449

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Physical maps. (A) Positions of the insertion sites of the E. coli replicon and the pyrEF marker genes for shuttle vectors pA–pN. (B): Conserved features of pRN1: thick arrows: conserved open reading frames, gray area: conserved on the nucleotide level within the pRN family plasmids, black arrows: transcripts. (C) Vector map of the shuttle construct pC. Positions of the restriction sites are (clockwise from the top): HindIII (1849), SacI (2792), HindIII (2833), SacII (5374), NotI (5380), SacI (6349).