Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Jun 18;35(12):e88. doi: 10.1093/nar/gkm449

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 5. — Replication of pJlacS in S. acidocaldarius (A) Southern blot (SacI) of plasmid pJlacS from E. coli, MR31 transformed with pJlacS and untransformed MR31 (−c). The pRN1 specific probe detects the 3.7-kb restriction fragment, the lacS probe detects the 7.3-kb fragment. (B) Retransformation of pJlacS (SacI), +c: pJlacS from E. coli, 1,2,3: retransformants. (C) X-gal test with untransformed MR31 and MR31 transformed with pJlacS (10 min at 75°C). (D) Growth curves for MR31 transformed with pJ (control) and with pJlacS. (E) Reporter gene experiment showing copy numbers of pJlacS per cell and the corresponding β-galactosidase activities.