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. 2007 Jun 18;35(12):e88. doi: 10.1093/nar/gkm449

Figure 6.

Figure 6.

Replication of pJlacS in S. solfataricus (A) Southern blot (SacI) of untransformed PBL2025 (−c), PBL2025 transformed with pJlacS and pJlacS from E. coli (+c). The pRN1-specific probe detects the 3.7-kb restriction fragment, the lacS probe detects the 7.3-kb fragment. (B) Growth curves of untransformed PBL2025 in 0.4% lactose medium, PBL2025 transformed with pJlacS in 0.4% lactose medium and in 0.2% tryptone medium. (C) Copy numper per cell for pJlacS in PBL2025 in 0.4% lactose medium. (D) Retransformation of pJlacS back into E. coli. (E) Plating of PBL2025 transformed with pJlacS after 100 generations of consecutive cultivation on non-selective tryptone and selective lactose plates stained with X-gal.