Table 1.
Insertion sites of the transposon in constructs pA–pN
| Plasmid | Direction of pyrEF genes | Insertion of transposon (position in pRN1) | Interruption | Adjacent unique restriction sites for cloning of inserts |
|---|---|---|---|---|
| pA | + | 1693|1694 | orf90b | NotI/SacII |
| pB | − | 1940|1939 | intergenic | NotI/XmaI |
| pC | + | 1778|1779 | orf90b | NotI/SacII |
| pD | + | 1758|1759 | orf90b | NotI/XmaI |
| pE | + | 1098|1099 | orf90a | NotI/SacII |
| pF | − | 5284|5283 | intergenic | NotI/SacII |
| pG | + | 5309|5310 | intergenic | NotI/SacII |
| pH | + | 621|622 | orf80 | NotI/SacII |
| pI | − | 5290|5289 | intergenic | NotI/XmaI |
| pJ | + | 12|13 | intergenic | NotI/SacII |
| pL | − | 1573|1572 | orf72 | NotI/XmaI |
| pM | + | 2334|2335 | orf56/orf904 | NotI/SacII |
| pN | + | 355|356 | intergenic | NotI/SacII |
Direction of the pyrEF genes (+ same direction as orf56/orf904) as well as two unique adjacent restriction sites are given.