. 2007 Jun 12;35(12):4076–4085. doi: 10.1093/nar/gkm434

Table 1.

Identification of PLSCR1 as a binding partner of the topo II α and β CTDs using yeast two-hybrid analysis

Plasmid combination	Growth^a
pOBD2/topo IIα1152-1531 + pACT/SNF4	–
pOBD2/topo IIβ1165-1621 + pACT/SNF4	–
pOBD2 + pACT/PLSCR1	–
pOBD2/topo IIα1152-1531 + pACT/PLSCR1	+
pOBD2/topo IIβ1165-1621 + pACT/PLSCR1	+
pOBD2/topo IIα1152-1531 + pACT/HDAC1220-482	−/+
pOBD2/topo IIβ1165-1621 + pACT/HDAC1220-482	+

^aYeast strain PJ69 was co-transformed with the plasmids as indicated and allowed to grow on interaction-selective plates. Growth was scored by presence of colonies after 5 days at 30°C. +, yeast growth; −, no growth.