Table 1.
Plasmid combination | Growtha |
---|---|
pOBD2/topo IIα1152-1531 + pACT/SNF4 | – |
pOBD2/topo IIβ1165-1621 + pACT/SNF4 | – |
pOBD2 + pACT/PLSCR1 | – |
pOBD2/topo IIα1152-1531 + pACT/PLSCR1 | + |
pOBD2/topo IIβ1165-1621 + pACT/PLSCR1 | + |
pOBD2/topo IIα1152-1531 + pACT/HDAC1220-482 | −/+ |
pOBD2/topo IIβ1165-1621 + pACT/HDAC1220-482 | + |
aYeast strain PJ69 was co-transformed with the plasmids as indicated and allowed to grow on interaction-selective plates. Growth was scored by presence of colonies after 5 days at 30°C. +, yeast growth; −, no growth.