Figure 1.

s-RT-MELT for rapid mutation scanning using enzymatic selection and real-time DNA-melting. (A) General outline of the approach. The dotted line contains the new steps involved in s-RT-MELT relative to previous approaches, i.e. the addition of a 3′-polynucleotide tail followed by real-time PCR that enables selective amplification of the Surveyor™-cut sequences and real time melting curve analysis. (B) Detailed outline of the procedure used to selectively amplify the mutation-containing fragments in s-RT-MELT.