Table 1.

Comparison of throughput in mutation scanning^a. Plus the ability to sequence and identify low-level somatic mutations

	One sample	16 samples (e.g. Cepheid QRT-PCR machine)	96 samples (96-well QRT-PCR machine)	384 samples (ABI QRT-PCR machine)	3,072 samples (OpenArray™)	Detects low-level mutations (1–10% mutant-to-wild type)	Nucleotide change and/or position of the mutation
dHPLC/Surveyorb	0.5 h	3-4 h	24 h	96 h	768 h	Yes	No
s-RT-MELTc	1.5 h	2.0 h	3.0 h	4.0 h	17 h	Yes	Yes

^aPost-PCR treatment time to accomplish pre-screening for unknown mutations.

^bSurveyor™ treatment requires ∼15–20 minutes. The dHPLC screens one sample at a time (12-15 minutes/sample including wash).

^cAn additional half hour for every batch of 96 samples purified via 96-sample Qiagen purification kit, following Surveyor™ treatment, was accounted for s-RT-MELT.