ASPA immunoreactivity in normal and Tremor mutant rat brain and kidney. A section of rat forebrain stained with anti-ASPA antibodies (1:5,000 dilution) is shown in A, whereas a similar section from a Tremor rat ASPA-null mutant is shown stained with the same antibody in B (1:1,000 dilution). In control rat kidney, ASPA expression was strong in proximal tubule cells (C; 1:5,000 dilution), but was not observed in glomeruli (arrows C and D). ASPA immunoreactivity was absent throughout the Tremor rat kidney (D; 1:2,000antibody dilution), Bar = 120 μm.