Figure 1.

Differential interactions of Kvβ1 and Kvβ2. HF7C yeast cells were transformed by pairwise combinations of the yeast two-hybrid constructs that express fusion proteins of either the DNA binding domain of GAL4 (DB) or the transcription activation domain of GAL4 (TA) (Right). Yeast transformants carrying the two fusion proteins were first selected by dextrose synthetic drop-out medium lacking leucine and tryptophan (Left) (SD, −leu, −trp, +his) to confirm that the transformants had taken both plasmids. An identical number of cells from each transformation were inoculated on the same medium lacking histidine (Left) (SD, −leu, −trp, −his). The cells were allowed to grow at 30°C for 48 h.