Figure 3.

Molecular determinants that mediate the Kvβ2–Kvβ2 interaction. (Left) A schematic diagram representing the coding sequence of Kvβ2 is shown with the C-terminal core region highlighted as a shaded box. Deletion constructs expressing different coding regions of Kvβ2 in GAL4–TA fusions are shown. The numbers in the parentheses indicate the beginning and ending amino acids of the individual deletion mutants. (Right) The ability of the deletion mutants to interact with full-length Kvβ2 or Kvβ2(39–367) was tested in the yeast two-hybrid system. Results from the growth assay are shown (+, growth; −, no growth).