Tables 3A and 3B. Particle size of rHDL species.
A. rHDL without SM. B. rHDL with SM. rHDLs were subjected to native gel electrophoresis, and sizes were measured by densitometry, as described in the Materials and Methods section. rHDL species in 3A and 3B, whose sizes are listed in grayed areas, have their corresponding electrophoretic profile displayed in Figures 3A and 3B, respectively.
| A
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B
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|---|---|---|---|---|---|
| Size distribution of rHDL without SM
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Size distribution of rHDL with SM
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| rHDL species | % PC | nm | rHDL species | PC:SM ratio | nm |
| DSPC:SM | |||||
| 250:0 | DSPC only | 6.9 | |||
| DSPC only | 100% DSPC | 6.9 | 200:50 | 80:20 | 6.9 |
| DSPC:DOPC | 150:100 | 60:40 | 6.8 | ||
| 50:250 | 20:80 | 6.9, 7.4 | |||
| 187.5:62.5 | 25% DOPC | 9.1, 8.1 | DSPC:DOPC:SM | ||
| 75:75 | 50% DOPC | 9.3 | 187.5:62.5:0 | 100:0 | 6.9, 7.7 |
| 150:50:50 | 80:20 | 6.9, 7.8 | |||
| 62.5:187.5 | 75% DOPC | 9.3 | 112.5:37.5:100 | 60:40 | 7.7 |
| 0:250 | 100% DOPC | 9.4 | 37.5:12.5:200 | 20:80 | 7.8 |
| DSPC:DLOPC | DSPC:DLOPC:SM | ||||
| 187.5:62.5:0 | 100:0 | 7.6 | |||
| 187.5:62.5 | 25% DLOPC | 8.8 | 150:50:50 | 80:20 | 7.6 |
| 112.5:37.5:100 | 60:40 | 7.6 | |||
| 75:75 | 50% DLOPC | 9.1 | 37.5:12.5:200 | 20:80 | 7.8, 8.6, 8.9 |
| 62.5:187.5 | 75% DLOPC | 9.0 | DSPC:DLNPC:SM | ||
| 0:250 | 100% DLOPC | 7.5 | 187.5:62.5:0 | 100:0 | 7.4 |
| 150:50:50 | 80:20 | 7.4 | |||
| DSPC:DLNPC | 112.5:37.5:100 | 60:40 | 7.4 | ||
| 37.5:12.5:200 | 20:80 | 9.4 | |||
| 187.5:62.5 | 25% DLNPC | 8.7, 7.5 | EYPC:SM | ||
| 75:75 | 50% DLNPC | 7.7, 6.3 | 250:0 | EYPC only | 8.9, 7.7 |
| 200:50 | 80:20 | 8.9, 7.7 | |||
| 62.5:187.5 | 75% DLNPC | 7.8, 6.4 | 150:100 | 60:40 | 9.0, 7.8 |
| 0:250 | 100% DLNPC | 8.0, 6.4 | 50:250 | 20:80 | 8.9, 7.8 |