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. Author manuscript; available in PMC: 2007 Jul 16.
Published in final edited form as: Cell. 2007 May 4;129(3):579–591. doi: 10.1016/j.cell.2007.03.023

Figure 6.

Figure 6

Spn-PP1-Dcx complex required for MT bundling during neurite outgrowth. (A) PP1γ knockdown associated with failure of MT bundling and broadened primary neurite. (C) Spn −/− splayed MT phenotype is rescued by forced expression of EGFP-tagged wildtype Spn, but not SpnΔCC (lacks Dcx binding) or Spn4A (lacks PP1 binding). (E) Dcx −/y splayed MT phenotype is rescued by forced expression of Dcx-RFP, but not by Dcx297A (unphosphorylatable) or Dcx297D (pseudophosphorylated) at the Spn-PP1 site. Scale bar 5 μm. (B, D, F) Significant difference in cells with bundled vs. splayed MT neurite phenotype. Results averaged from two experiments. * = p < 0.01, Student t-test.