. 2007 May 5;35(11):3525–3534. doi: 10.1093/nar/gkm142

Table 3.

Binding characteristics of the IRF-3 N-terminal domain (NTD), full-length IRF-3 WT and its 2D and 5D mutants with the 26-bp DNA containing the PRDIII- and PRDI-binding sites, and with the 13-bp DNA containing the single PRDI site

Protein	26-bp DNA containing the PRDIII-PRDI-binding sites		13-bp DNA containing the PRDI-binding site

	K_d (nM)	ΔG^a (kJ/mol)	K_d (nM)	ΔG^a (kJ/mol)
NTD	–	–	280 ± 15	36.7 ± 0.2
IRF-3 WT	1226 ± 15	33.1 ± 0.1	800 ± 17	34.1 ± 0.1
IRF-3 2D	1300 ± 15	33.0 ± 0.1	900 ± 15	33.8 ± 0.1
IRF-3 5D, dimer	5.8 ± 0.8	46.1 ± 0.3	1200 ± 20	33.1 ± 0.1

Binding buffer: 100 mM NaCl, 30 mM Na-phosphate buffer, pH 7.4. 2D and 5D are the phosphomimetic mutants of WT IRF-3 in which two residues, S396 and S398, and five residues, S396, S398, S402, T404 and S405, respectively are substituted by Asp residues. IRF-3 NTD is the N-terminal DNA-binding domain of the IRF-3 protein. Binding parameters are calculated per mole of IRF-3 monomer.