Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 May 5;35(11):3535–3550. doi: 10.1093/nar/gkm195

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 5. — (A) Cell cycle progression of stable knock-down HeLa clones after UVC (10 J/m²), γ rays (6 Gy) or etoposide treatment (VP16; 25 μM for 1.5 h): GG-NER and TC-NER pathways. Exponentially growing cells were treated and 24 h later adherent cells were collected, washed and fixed in 75% ethanol at 4°C for at least 24 h. Cells were stained with propidium iodide (4 μg/ml) in the presence of RNase (10 μg/ml) for at least 30 min. Stained cells were analyzed on a FACScalibur (Becton Dickinson) using CellQuest software. Here, ∼10 000 cells gated as single cells using FL2A/FL2W scatter were analyzed. Each clone was analyzed at least three times. Long-term HeLa clones: Control (BD650, day115), XPC^KD (BD634/21; day 333), hHR23A^KD (BD805/7; day 158), hHR23B^KD (BD804/9; day 191), XPA^KD (BD695/6; day 422). Arrows indicate a striking point highlighting intolerance to DNA damage. (B) NHEJ and HR pathways. Long-term HeLa clones: DNAPKcs^KD (BD743/1; day 321), XRCC4^KD (BD694/9; day 235), LigIV^KD (BD940/3; day 103), LigIII^KD (BD941/11; day 82), Rad51^KD (BD864/2; day 107), Rad54^KD (BD912/5; day 148). (C) Signaling pathways Long-term HeLa clones: MRE11^KD (BD930/32; day 61), Rad50^KD (BD872/1; day 158), NBS1^KD (BD932/11; day 108), ATM^KD (BD935/12; day 111), ATR^KD (BD962/9; day 56).

Figure 5. — (A) Cell cycle progression of stable knock-down HeLa clones after UVC (10 J/m²), γ rays (6 Gy) or etoposide treatment (VP16; 25 μM for 1.5 h): GG-NER and TC-NER pathways. Exponentially growing cells were treated and 24 h later adherent cells were collected, washed and fixed in 75% ethanol at 4°C for at least 24 h. Cells were stained with propidium iodide (4 μg/ml) in the presence of RNase (10 μg/ml) for at least 30 min. Stained cells were analyzed on a FACScalibur (Becton Dickinson) using CellQuest software. Here, ∼10 000 cells gated as single cells using FL2A/FL2W scatter were analyzed. Each clone was analyzed at least three times. Long-term HeLa clones: Control (BD650, day115), XPC^KD (BD634/21; day 333), hHR23A^KD (BD805/7; day 158), hHR23B^KD (BD804/9; day 191), XPA^KD (BD695/6; day 422). Arrows indicate a striking point highlighting intolerance to DNA damage. (B) NHEJ and HR pathways. Long-term HeLa clones: DNAPKcs^KD (BD743/1; day 321), XRCC4^KD (BD694/9; day 235), LigIV^KD (BD940/3; day 103), LigIII^KD (BD941/11; day 82), Rad51^KD (BD864/2; day 107), Rad54^KD (BD912/5; day 148). (C) Signaling pathways Long-term HeLa clones: MRE11^KD (BD930/32; day 61), Rad50^KD (BD872/1; day 158), NBS1^KD (BD932/11; day 108), ATM^KD (BD935/12; day 111), ATR^KD (BD962/9; day 56).