Figure 2.

DNase I footprint analysis of the A2 region of the GmCaM-4 promoter bound by pathogen-responsive proteins. End-labeled fragments were digested with DNase I after incubation with 0, 5, 10, 20 or 25 μg nuclear proteins from Psg. avrA-treated W82 soybean cells. The resulting DNA fragments were purified and electrophoresed on a 6% non-denaturing polyacrylamide sequencing gel. GA, G + A sequencing ladder (non-coding strand); lane 1, no nuclear protein; lanes 2–5, 5, 10, 20 and 25 μg nuclear protein, respectively. The DNase I-protected region is indicated by a solid bar and the sequence is shown.