Figure 5.

Effects of the cycle number on the hierarchical oligonucleotide primer extension method. The reactions are carried out using the thermal cycling conditions: denaturation at 96°C for 30 s, annealing at 60°C for 30 s and extension at 72°C for 30 s. The experiments are conducted using Bacteroides thetaiotaomicron 16S rRNA gene amplicons and the primer mixture containing EUB338Ia, BAC303-5a, BTH274-15a, and BTH584-16a. The data points represent the average values derived from two independent reactions. The error bars represent standard deviation for average data points.