Table 3.
Quantitative analyses of the model communities composed of defined 16S rRNA gene amplicons using multiplexing HOPE method.
| Model communities | Concentration of 16S rRNA gene amplicons prepared in the model communitiesa | % of primers for specific groups with respect to primer EUB338Ia for domain Bacteria (mean ± sd)b | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| B. thetaiotaomicron | B. distasonis | B. vulgatus | L. acidophilus | E. faecium | P. productus | E. coli | M. barkeric | BAC303-5a (for Bacteroides) | BTH274-15a (for B. thetaiotaomicron and B. fragilis) | BTH584-16a (for B. thetaiotaomicron) | ||||
| Theoretical | Observed | Theoretical | Observed | Theoretical | Observed | |||||||||
| MC1 | 2.2 (22.1%) | 2.5 (24.8%) | 2.5 (25.7%) | 2.7 (27.3%) | 0 | 0 | 0 | 0 | 72.7 | 77.0 ± 3.9 | 22.1 | 18.4 ± 0.7 | 22.1 | 18.5 ± 1.2 |
| MC2 | 2.2 (21.8%) | 2.5 (24.4%) | 0 | 2.7 (26.8%) | 2.7 (26.9%) | 0 | 0 | 0 | 46.2 | 49.2 ± 3.6 | 21.8 | 17.6 ± 0.7 | 21.8 | 18.1 ± 1.4 |
| MC3 | 2.2 (21.5%) | 0 | 0 | 2.7 (26.5%) | 2.7 (26.6%) | 2.7 (25.3%) | 0 | 0 | 21.5 | 14.8 ± 1.0 | 21.5 | 14.4 ± 0.9 | 21.5 | 14.9 ± 1.0 |
| MC4 | 1.1 (5.2%) | 1.2 (5.9%) | 0 | 1.3 (6.5%) | 1.4 (6.5%) | 0 | 15.8 (75.9%) | 0 | 11.1 | 9.7 ± 0.8 | 5.2 | 2.8 ± 0.3 | 5.2 | 3.4 ± 1.0 |
| MC5 | 1.1 (21.8%) | 1.2 (24.4%) | 0 | 1.3 (26.8%) | 1.4 (26.9%) | 0 | 0 | 12.8 (0%) | 46.2 | 53.1 ± 2.9 | 21.8 | 17.4 ± 0.4 | 21.8 | 15.3 ± 1.2 |
afemto-mol/µl; percentages in the brackets indicate species abundance in the Bacteria domain.
bn = 6.
cMethanosarcina barkeri (DSMZ 1538).