Figure 6.

Effect of 5-HT on the phosphorylation of an apCAM-derived peptide by fractionated MAPK. Kinase activity against apCAM peptides was determined in control extracts or extracts from 5-HT-treated ganglia. Paired pleural-pedal ganglia were incubated in the absence (− 5-HT) or presence of 20 μM 5-HT for 30 min and cell extracts were subjected to fractionation on anion-exchange FFQ minicolumns. Samples from the individual fractions were allowed to phosphorylate apCAM-derived peptide. This experiment was repeated (n = 3) and quantitation (mean percentage change in fractions F7 and fraction F8, combined) revealed that 5-HT caused an increase in kinase activity against apCAM by 73% ± 4% (SE) (P < 0.01).