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. Author manuscript; available in PMC: 2007 Jul 16.
Published in final edited form as: Exp Cell Res. 2005 Oct 10;311(1):39–48. doi: 10.1016/j.yexcr.2005.08.020

Fig. 1.

Fig. 1

Effect of TNF-α on MAK kinase activation in HCE cells. (A) Dose-dependent effects of TNF-α stimulation on JNK activity. TNF-α was added into HCE cells at different concentrations. UV irradiation was used to activate JNK as a positive control. JNK activation was analyzed by Western blots using Western analysis with antibodies against phospho-JNK 30 min after the stimulation. (B) Effect of TNF-α stimulation on Erk activity. Effects of TNF-α stimulation on Erk and JNK activation were measured at indicated time points using Western analysis with antibodies against phospho-JNK and phospho-Erk. (C) Effect of TNF-α stimulation on p38 activity. Effects of TNF-α stimulation on p38 and JNK activation with/without UV irradiation were detected following a time course. HCE cells were incubated with 20 mg/ml TNF-α for different incubation periods. Whole cell lysates from UV exposed HCE cells were used as a positive control of phospho-JNK and phospho-p38.