Table 2.
cDNAs identified as differentially regulated by microarray analysis. Clone ID1 is the accession number of the cDNA feature on the microarray, when more than one cDNA feature was the same sequence the accession number for the feature with the highest fold difference is given, the number in brackets indicates the number of features identical and used in analysis. Identity2 is the functional protein assigned to the cDNA feature, Accession3 number relates to the identify of the protein. *indicates cDNA features where protein function is determined from NCBI UniGene as described in the text. Mean is the mean fold difference between the stimulated and non stimulated control cells.
| Clone ID1 | Identity2 | Accession3 | Gene Ontology Identifier | Mean | SEM |
| Up regulated by rIFN-γ | |||||
| CA063565(2) | Interferon regulatory factor 1 | AAM77843 | GO:0003700: transcription factor activity | 12.1 | 4.9 |
| CA062585(2) | Tapasin | ABE27285 | GO:0019882: antigen presentation | 11.4 | 3.5 |
| CA063956 | Complement C1q | XP_544507.2 | GO:0006955: immune response | 11.4 | 2.7 |
| CB505764 | C1q-like adipose specific protein | AAM73701 | GO:0006955: immune response | 10.6 | 5.8 |
| CA062838*(2) | Acyl-coenzyme A-binding protein | AJ632152 | GO:0006810: transport | 10.3 | 1.4 |
| CA051393* | Interferon consensus sequence binding protein (IRF-8) | NP_990747.1 | GO:0003700: transcription factor activity | 10.0 | 4.7 |
| CA062698* | Ssa.15825 | N/A | 9.6 | 5.0 | |
| CB506130 | Proteasome subunit beta type 1-A 20S | O09061 | GO:0006511: ubiquitin-dependent protein catabolism | 9.2 | 6.2 |
| CA062737(3) | Low molecular mass protein 7 | P28063 | GO:0006955: immune response | 8.8 | 3.3 |
| CA052500 | TAP 1 | Q9JJ59 | GO:0019882: antigen presentation | 8.7 | 1.4 |
| CA056108(2) | C-type lectin 2-1 | Q91ZW7 | GO:0006955: immune response | 8.5 | 3.8 |
| CA055080 | Core histone macroH2A2.2; H2A histone family | AAH76893 | GO:0044237 : cellular metabolism | 7.6 | 4.3 |
| CB516220(2) | Inhibitor of nuclear factor kappa B alpha | Q9Z1E3 | GO:0006955: immune response | 7.3 | 3.9 |
| CA057910* | Programmed cell death 1 ligand 1 | XP_424811.1 | GO:0008219 : cell death | 6.8 | 1.5 |
| CA051372(2) | Low molecular mass protein 2 | Q60692 | GO:0006955: immune response | 6.5 | 2.5 |
| CB511048 | VHSV-induced C-lectin-like protein | AY572832 | GO:0006955: immune response | 6.1 | 3.0 |
| CA063009*(2) | Ssa.6457 | N/A | 6.0 | 1.7 | |
| CA052717* | Fc receptor-like 4 | XP_547521.2 | N/A | 5.7 | 2.0 |
| CA050149 | IMMUNE-RESPONSIVE PROTEIN 1 | XP_542615 | N/A | 4.1 | 1.6 |
| CA050971 | Proteasome subunit alpha type 6 | Q9QUM9 | GO:0006511: ubiquitin-dependent protein catabolism | 3.8 | 1.5 |
| CA063863* | Similar to interferon regulatory factor 1 | NP_002189.1 | GO:0003700: transcription factor activity | 3.6 | 0.4 |
| CA052613 | Ubiquitin conjugating enzyme | XP_517968 | GO:0006511: ubiquitin-dependent protein catabolism | 3.6 | 0.6 |
| CB500108 | Ribosomal protein L35 | AAM34649 | GO:0006412: protein biosynthesis | 3.4 | 0.8 |
| CA044026(2) | MHC class I | L07605 | GO:0019882: antigen presentation | 3.4 | 0.4 |
| CA052774 | Protein phosphatase 2 | P62715 | GO:0044237 : cellular metabolism | 3.0 | 0.5 |
| CB511680(2) | Lysozyme | P17897 | GO:0006952: defense response | 3.0 | 0.3 |
| CA055186 | MECL1 | P70195 | GO:0006511: ubiquitin-dependent protein catabolism | 2.7 | 0.5 |
| CB493732 | Proteasome subunit beta type 7 | NP_989728.1 | GO:0006511: ubiquitin-dependent protein catabolism | 2.7 | 0.7 |
| CA044982 | HES1 | Q9D172 | N/A | 2.6 | 0.7 |
| CB496534(3) | Ferritin | P09528 | GO:0006955: immune response | 2.3 | 0.1 |
| CA057391(2) | Granulin | XP_537620.2 | GO:0008283: cell proliferation | 2.2 | 0.4 |
| CA060176 | Galectin like protein | O08573 | GO:0006952: defense response | 2.2 | 0.3 |
| CA060021 | Unknown | N/A | 2.2 | 0.2 | |
| CA046740 | Sequestosome 1; oxidative stress induced | NP_035148 | GO:0007165: signal transduction | 2.1 | 0.1 |
| Down regulated by rIFN-γ | |||||
| CA057408 | Annexin A2 | P07356 | GO:0050819 : negative regulation of coagulation | 3.0 | 0.9 |
| CB492780(2) | Id2 protein | P41136 | GO:0045449: regulation of transcription | 2.9 | 0.4 |
| CA040487* | Ssa.17039 | N/A | 2.4 | 0.2 | |
| CK990422* | Ssa.2967 | N/A | 2.3 | 0.4 | |
| CA769647* | Ssa.3973 | N/A | 2.3 | 0.2 | |
| CB509992 | 18S ribosomal RNA gene, partial sequence | AY856868 | N/A | 2.1 | 0.1 |
| CB491705 | similar to PREDICTED: similar to ORF2 | XP_425603.1 | N/A | 2.1 | 0.4 |
| CB489453 | Hypothetical protein 2 | XP_417016 | N/A | 1.9 | 0.1 |
| Up regulated by rIL-1β | |||||
| CA050149 | IMMUNE-RESPONSIVE PROTEIN 1 | XP_542615 | N/A | 27.7 | 10.4 |
| CB511048 | VHSV-induced C-lectin-like | AY572832 | GO:0006955: immune response | 23.2 | 10.8 |
| CA061271 | UNKNOWN | N/A | 21.2 | 9.8 | |
| CK991068(3) | Hepcidin | AAO85553 | GO:0006952: defense response | 21.2 | 5.0 |
| CA055080 | Core histone macroH2A2.2; H2A histone family | AAH76893 | GO:0044237: cellular metabolism | 20.7 | 2.2 |
| CA037550 | 60S ribosomal protein L30 | P62889 | GO:0006412: protein biosynthesis | 19.0 | 8.5 |
| CB496842(3) | C-type lectin | Q91ZW7 | GO:0006955: immune response | 18.4 | 4.1 |
| CA054326 | Tax1 binding protein 1 | NP_006015.4 | GO:0007165: signal transduction | 15.9 | 10.5 |
| CA063656 | Cyclin L1 | AY606031 | N/A | 14.2 | 10.4 |
| CB511230 | TAP2 | AF002180 | GO:0019882: antigen presentation | 13.0 | 6.4 |
| CK990725 | RhoE | P61588 | GO:0007165: signal transduction | 12.5 | 5.6 |
| CB504496(3) | Differentially regulated trout protein 1 | AAG30030 | GO:0006955: immune response | 12.3 | 4.5 |
| CA063738* | Nuclear factor of k light polypeptide gene enhancer (p49/p100) | NP_989744. | GO:0007165: signal transduction | 12.2 | 2.9 |
| CB498152* | Similar to MARCKS-like 1 | NP_075385.1 | GO:0005516: calmodulin binding | 12.2 | 3.2 |
| CK990269* | Connexin 31 | NP_990262.1 | GO:0007154: cell communication | 12.1 | 2.4 |
| CB516988* | PREDICTED: similar to heparan sulfate proteoglycan | XP_417362.1 | GO:0006917: induction of apoptosis | 12.1 | 4.4 |
| CB512516* | Ssa.2559 | N/A | 12.1 | 9.0 | |
| CB516929* | Ssa.5205 | N/A | 12.0 | 4.7 | |
| CA060421(2) | CD83 | AAP93912 | GO:0006952: defense response | 11.5 | 4.8 |
| CB514313 | Delta 1 | Q61483 | GO:0007154: cell communication | 11.1 | 7.2 |
| CA063097* | Ssa.6200 | N/A | 11.1 | 3.6 | |
| CB511158(2) | Precerebellin-like protein | AAF04305 | GO:0006952: defense response | 10.7 | 5.3 |
| CA041210 | MARCKS-like protein | P28667 | GO:0005516: calmodulin binding | 9.8 | 1.7 |
| CA059788(3) | Inhibitor of nuclear factor kappa B alpha | AAH62524 | GO:0006955: immune response | 9.6 | 2.6 |
| CA044647* | Ssa.3390 | N/A | 9.6 | 0.9 | |
| CA060755*(2) | Ssa.2872 | N/A | 9.4 | 2.3 | |
| CA051323* | Ssa.6734 | N/A | 9.3 | 5.0 | |
| CA053062(3) | 5-aminolevulinic acid synthase | Q8VC19 | GO:0009058: biosynthesis | 8.3 | 2.6 |
| CA061251 | RAS-related C3 botulinum substrate 2 | Q05144 | GO:0006952: defense response | 8.2 | 4.6 |
| CB507662* | Ssa.383 | N/A | 8.1 | 0.4 | |
| CB501098* | Ssa.3941 | N/A | 7.9 | 4.4 | |
| CA058223 | Guanylate binding protein 4 | Q01514 | GO:0006955: immune response | 7.8 | 2.8 |
| CB515854* | Similar to tumor necrosis factor, alpha-induced protein 2 | NP_006282.2 | GO:0006952: defense response | 7.8 | 1.1 |
| CA052773 | Arginyl-tRNA synthetase | Q9D0I9 | GO:0009058: biosynthesis | 7.2 | 4.5 |
| CA050427*(3) | Ssa9019 | N/A | 7.2 | 1.6 | |
| CB498855(2) | 6-phosphofructo-2-kinase | P70265 | GO:0044237: cellular metabolism | 7.0 | 1.2 |
| CA046850 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 | P17844 | GO:0016049: cell growth | 6.8 | 3.7 |
| CA056715(3) | Transcription factor JUN-B | P09450 | GO:0045449: regulation of transcription | 6.5 | 0.7 |
| CA063635* | Ssa.5016 | N/A | 6.5 | 3.4 | |
| CB514104 | Adipophilin (Adipose differentiation-related protein) | P43883 | GO:0006810: transport | 6.3 | 0.7 |
| CA038364 | Similar to ubiquinol-cytochrome c reductase complex | P00130 | GO:0044237: cellular metabolism | 6.2 | 1.6 |
| CA054114* | Ssa.25362 | N/A | 6.2 | 1.6 | |
| CA770328 | Tapasin | ABE27285 | GO:0019882: antigen presentation | 5.8 | 1.5 |
| CA054633 | ATP-binding cassette transporter 2 | P21440 | GO:0006810: transport | 5.5 | 1.7 |
| CB501435* | Omy.30821 | N/A | 5.4 | 0.6 | |
| CA055219(2) | CCAAT/enhancer binding protein beta, | AAH49401 | GO:0045449: regulation of transcription | 5.0 | 0.8 |
| CA062875 | Similar to IFN consensus sequence binding protein IRF-8 | Q90871 | GO:0045449: regulation of transcription | 5.0 | 1.4 |
| CB517430 | Similar to epithelial stromal interaction 1 | NP_150280.1 | N/A | 4.9 | 1.5 |
| CA053490 | Tumour necrosis factor receptor | CAD57165 | GO:0007165: signal transduction | 4.8 | 1.1 |
| CK990939 | Adipose differentiation-related protein | Q9MZE5 | GO:0016020: membrane | 4.7 | 0.5 |
| CA051393(2) | Similar to interferon consensus sequence binding protein | NP_990747.1 | GO:0045449: regulation of transcription | 4.7 | 1.2 |
| CB511966 | Factor VIII | Q06194 | GO:0006952: defense response | 4.4 | 0.7 |
| CA043801 | Ssa.22345 | N/A | 4.4 | 1.2 | |
| CA062585 | TAPBP protein... | Q8UUL4 | GO:0019882: antigen presentation | 4.4 | 0.9 |
| CA053654* | Ssa.7103 | N/A | 4.3 | 0.9 | |
| CA060783 | Similar to protein tyrosine phosphatase | NP_035336.1 | GO:0006464: protein modification | 4.2 | 1.1 |
| CB515947 | Ssa.13995 | N/A | 4.2 | 1.0 | |
| CB515614 | Ssa.4774 | N/A | 4.1 | 1.2 | |
| CB497913 | Omy.8396 | N/A | 3.9 | 0.4 | |
| CB498900 | Ssa.14843 | N/A | 3.9 | 1.0 | |
| CA767815 | sequestosome 1 | NP_035148 | GO:0007165: signal transduction | 3.8 | 0.4 |
| CA056501 | Serine (or cysteine) proteinase inhibitor | Q07235 | N/A | 3.7 | 0.7 |
| CA044867 | Ssa.8481 | N/A | 3.7 | 1.0 | |
| CB494479 | Transcription factor ETV6 | P97360 | GO:0045449: regulation of transcription | 3.7 | 0.3 |
| CA063565 | Interferon regulatory factor 1 | AAM77843 | GO:0045449: regulation of transcription | 3.6 | 0.7 |
| CB496486(2) | Low molecular mass protein 7 | P28063 | GO:0006955: immune response | 3.5 | 0.6 |
| CB498525 | Secretory granule proteoglycan core protein | XP_507828.1 | N/A | 3.5 | 0.7 |
| CB515392 | Stomatin | P54116 | GO:0007165: signal transduction | 3.5 | 0.2 |
| CA052717 | similar to Fc receptor-like 4 | XP_547521.2 | N/A | 3.4 | 0.2 |
| CA052500 | TAP1A | Q9JJ59 | GO:0019882: antigen presentation | 3.4 | 0.2 |
| CB494116 | UNKNOWN | N/A | 3.3 | 0.8 | |
| CB489126 | Protein translation factor SUI1 | Q9CXU9 | GO:0006412: protein biosynthesis | 3.2 | 0.3 |
| CK990998(3) | similar to Protein translation factor SUI1 homolog | P48024 | GO:0006412: protein biosynthesis | 3.2 | 0.3 |
| CA062698 | Ssa.15825 | N/A | 3.2 | 0.4 | |
| CB499179 | Leucine-rich-repeat protein | XM_039906 | GO:0006952: defense response; | 3.1 | 0.4 |
| CK991328 | Omy.3531 | N/A | 3.1 | 1.0 | |
| CA062450* | Similar to melanoma cell adhesion molecule | NP_075548.1 | GO:0007155: cell adhesion | 3.1 | 0.5 |
| CA051372 | Low molecular mass protein 2 | Q60692 | GO:0006955: immune response | 3.0 | 0.9 |
| CA057910* | Similar to programmed cell death 1 ligand 1 | XP_424811.1 | GO:0006955: immune response | 3.0 | 0.4 |
| CA056074 | p60 | AAH01874 | GO:0007165: signal transduction | 2.9 | 0.3 |
| CB488180 | ATP synthase | Q9DCX2 | GO:0015986: ATP synthesis coupled proton transport | 2.9 | 0.2 |
| CA052868 | NADPH oxidase cytosolic protein p40phox | AB192468 | GO:0007165: signal transduction | 2.9 | 0.3 |
| CA043996* | Ssa.3435 | N/A | 2.8 | 0.3 | |
| CB503379* | Ssa.7962 | N/A | 2.8 | 0.2 | |
| CB511558 | UNKNOWN | N/A | 2.7 | 0.3 | |
| CA060176 | Galectin like protein | O08573 | GO:0006952: defense response | 2.6 | 0.2 |
| CB502697* | Transmembrane 4 superfamily member 3 | NP_598210.1 | N/A | 2.6 | 0.3 |
| CB498077(4) | Ferritin | P09528 | GO:0006955: immune response | 2.5 | 0.1 |
| CA039745 | Alpha-globin | CAA65955 | GO:0006810: transport | 2.5 | 0.2 |
| CA062784 | UNKNOWN | N/A | 2.5 | 0.2 | |
| CB493123 | Activating transcription factor 4, | Q6T3V3 | GO:0045449: regulation of transcription | 2.4 | 0.3 |
| CB488782 | Growth regulated nuclear 68 protein | Q61656 | GO:0045449: regulation of transcription | 2.3 | 0.1 |
| Down regulated by IL-1β | |||||
| CA056515 | UNKNOWN | N/A | 5.4 | 2.8 | |
| CA041370 | PREDICTED: similar to MAFB protein | XP_417353.1 | GO:0045449: regulation of transcription | 4.7 | 1.7 |
| CA062828(3) | Id2 protein | P41136 | GO:0045449: regulation of transcription | 4.0 | 0.4 |
| CB514524 | xCDC46 | BAA09949 | GO:0045449: regulation of transcription | 3.3 | 0.4 |
| CB517923 | Transforming protein myc | P01108 | GO:0045449: regulation of transcription | 3.1 | 0.7 |
| CB502538 | UNKNOWN | N/A | 2.6 | 0.7 | |
| CA052685 | Ribonucleotide reductase protein r2 class I | P11157 | GO:0044237 : cellular metabolism | 2.4 | 0.2 |