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. 2007 Mar 26;8(5):486–499. doi: 10.1111/j.1600-0854.2007.00548.x

Figure 3. Efficiency of shRNAs to KD Myrip expression.

Figure 3

A) HEK-293FT cells were co-transfected with pENTR/CMV-V5-Myrip and pENTR/U6-shRNA. Protein extracts were subjected to immunoblot using anti-V5 antibody. B) Wild-type RPE cells were fixed, permeabilized and subjected to immunofluorescence using anti-Myrip antibody. Wild-type RPE cells expressing no GFP-shRNA (panels A–C), GFP-Myrip shRNA5 (panels D–F) and GFP non-specific shRNA4 (panels G–H) were labelled with Myrip antibody, as indicated. Bar = 20 μm.