Figure 1. PCR and western blot analysis for parkin expression in wild-type, heterozygotes and parkin knockout mice.

(A) PCR analysis of genomic DNA showing various genotypes of mice, i.e. wild-type, heterozygotes and parkin knockout mice. (B) To assess expression of parkin in wild-type (WT), heterozygous (hets), and knockout (KO) mice, total protein isolated from 6-month-old whole brains was subjected to immunoblot analysis for parkin with a monoclonal parkin antibody.