Figure 1.
Effect of kainic acid on the expression of NDF/neuregulin and its ErbB-3 and ErbB-4 receptors in rat brain. Coronal brain sections were prepared from rats 4.5 hr after intraperitoneal injection of kainate (KA) and hybridized to the following 35S-labeled cRNA probes: NDF (A), erbB-3 (B), and erbB-4 (C). Dark-field photomicrographs are shown in comparison with sections that were prepared from brains of untreated rats (CONTROL). The far-right panels show high magnification of cells in the hippocampal areas CA1 and CA3. Note the punctate appearance of ErbB-4 staining corresponding to single cells especially in control brains. A, amygdala; CA1 and CA3, fields of Ammon’s horn; DG, dentate gyrus; Hy, hypothalamus; Rt, reticular thalamic nucleus; Pir, piriform cortex; and Th, thalamus. Scales in C also are for the corresponding images in A and B.