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. 1998 Feb 17;95(4):1894–1898. doi: 10.1073/pnas.95.4.1894

Figure 3.

Figure 3

Reversal of IGFBP-2 inhibition of hIGF-I-stimulated fibroblast proliferation by [Leu24,59,60, Ala31]hIGF-I in vitro. Human IGF-I dose-dependently stimulated DNA synthesis with an ED50 of 5–10 nM. In contrast, [Leu24,59,60, Ala31]hIGF-I did not induce DNA synthesis in 3T3 cells at any of the doses tested (0.1–8,000 nM). IGFBP-2 (20 nM) substantially inhibited the proliferative effect of 3 nM hIGF-I. Addition of IGFBP ligand inhibitor dose-dependently reversed this inhibition with an ED50 of 200 nM.