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Tissue distribution of the two Δ7-sterol reductase transcripts. Northern Blots with 2 μg of poly(A)+ RNA (Multiple tissue Northern blot, CLONTECH) were probed with the 32P-labeled human Δ7-sterol reductase (A) and human sterol Δ8-Δ7 isomerase cDNAs (B), respectively, and exposed for 16 hr. The arrows indicate the migration of the 2.9-kb and 2.3-kb Δ7-sterol reductase (A) and the 1.3-kb sterol isomerase (B) mRNAs, respectively.
