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Requirement of de novo synthesis for the recovery of the Na/Pi cotransport from PTH inhibition. OK cells have been preincubated for 30 min with (B) or without (A) leupeptin (100 μg/ml) followed by an incubation for 2 h with PTH (10−8 M) in the continued presence (B) or absence (A) of leupeptin. Thereafter cells were washed twice with normal medium and incubated for 4 h with (○) or without (▵) cycloheximide (100 μM) in the continued presence (B) or absence (A) of leupeptin.
