Figure 3.

Summary of channel activity in the presence of a 1,000-fold concentration gradient for S-Oligo. (A) Representative 5-s traces from an experiment obtained at different holding potentials in the presence of a 1,000-fold S-Oligo gradient (500 μM S-Oligo cis and 0.5 μM S-Oligo trans). The solid horizontal line in each trace indicates the closed state. (B) Current–voltage relationship summarizing five experiments in which the presence of an oligonucleotide current was measured after establishing a 1,000-fold concentration gradient for S-Oligo. Conductance of the channel was 10.2 ± 0.7 pS and there was a substantial shift in reversal potential to +28.5 ± 2.8 mV. This shift in reversal potential is consistent with movement of oligonucleotide through the channel.