Figure 3.

MP2C encodes an active PP2C. The MP2C gene was expressed as a GST fusion protein in E. coli. Affinity-purified GST–MP2C was incubated with ³²P-labeled casein, and the released ³²P_i was taken as a measure for the phosphatase activity. ³²P_i released by incubation of ³²P-labeled casein with GST containing Mg²⁺ (bar 1), GST–MP2C containing Mg²⁺ (bar 2), GST–MP2C containing Mn²⁺ (bar 3), GST–MP2C containing Ca²⁺ (bar 4), GST–MP2C containing Mg²⁺ and 10 mM EDTA (bar 5), GST–MP2C containing Mn²⁺ and 10 mM EDTA (bar 6) is shown.