Figure 4.
Expression of the SQD1 gene in leaves of A. thaliana. (A) Wild type, the strongest SQD1 antisense line AS1, and the pho1 mutant grown for 14 days in soil. (B) Wild type germinated and raised for 7 days on agar-solidified Murashige–Skoog medium and subsequently grown for 7 days on mineral medium containing a concentration of Pi (mM) as indicated. (Upper) SQD1 mRNA (SQD1) or the presumed antisense RNA (asSQD1) detected by hybridization with the SQD1 cDNA (the approximate sizes are indicated). (Middle) SQD1 protein (SQD1) detected by immunoblotting (the apparent molecular mass is indicated). (Lower) Central part of iodine-stained lipid chromatograms (DGDG, digalactosyl diacylglycerol; PE, phosphatidylethanolamine; SQDG, sulfoquinovosyl diacylglycerol). RNA, protein, and lipids were isolated from identical batches of plants. Equal amounts of total RNA, protein, or lipids were loaded based on visual examination of stained gels or TLC plates.