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. Author manuscript; available in PMC: 2007 Jul 17.
Published in final edited form as: J Comp Neurol. 2001 Jul 30;436(3):336–350.

Fig. 2.

Fig. 2

Western blot analysis shows that Zymed anti-Cx36 antiserum recognizes rabbit Cx36. A portion of the rabbit Cx36 cDNA coding for the intracellular loop (amino acids 94-198) was subcloned into a GST expression vector (pGex2KG) and expressed in Escherichia coli. Here, a crude bacterial lysate was blotted, probed with the Cx36 antibody (1:5000), and detected by chemiluminescence.